GPR83: A Novel Treg Expressed Cell Surface Marker

GPR83 is a member of the Orphan-A G-Protein coupled receptor family, and has anunknown ligand. It has been previously reported in various regions of the brain, within a subset of T-lymphocytes, and by RT-PCR at low levels in heart, kidney, liver, and otherorgans.

As research continues to unravel the molecular basis of regulatory T cells (Treg), FOXP3 has emerged as a key player in orchestrating development and function of natural Treg. Two recent publications identified GPR83 as specifically upregulated in fresh and activated CD4+ CD25+ Treg cells. Both groups performed gene expression profiling on Treg, and other T cells to identify differentially regulated genes. Sugimoto et al. performed additional mRNA and protein expression analysis based on the results of their micro array analysis using RT-PCR as well as FACS analysis of GPR83 using anti-GPR83 antibody. These recent findings suggest that GPR83 may be useful as a specific and stable cell surface marker with modulatory properties, and a key molecule for further characterizing the molecular basis of Tregs. IMGENEX proudly offers this Flow and IHC positive GPR83 antibody (IMG-71561), as well as other GPR83, FOXP3, and CD marker antibodies for your Treg research.

Key Publication Findings
•GPR83 is upregulated in both murine and human Treg • Gene analysis, mRNA, and protein expression show that GPR83 is upregulated in FOXP3+ cells
• GPR83 is expressed in both fresh and activated Treg
• GPR83 appears to be FOXP3 dependent, although Hansen et al. suggests that induction of FOXP3+
Treg by GPR83 occurs in vivo, suggesting a more complex relationship
• GPR83 is found in both Thymus and Spleen/LN CD4 and CD25 cells
• Foxp3 retroviral transduced CD25- CD4+ cells induces GPR83 expression at much higher levels than mock-transduced cells
• Suggests a possible ligand that binds to GPR83 in vivo which confers to these cells suppressive activity.

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